Both the temporal and spatial expression of genomes is influenced by chromatin topology, which is governed by conserved processes. These mechanisms have been implicated in numerous adaptations, including the response to biotic and abiotic stresses as well as in developmental programs. But they are still many open questions that we are tackling now: What determine chromatin architecture?How are promoter-enhancer contacts established in plants? How are these contacts established and released over time? What is the function of TFs in promoter-enhancer contact formation and or stability? What are the molecular mechanisms behind it? In this context to answer those questions we use genetics, genomics and epigenomics approaches.